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TL;DR
The study isolated a chitinase gene (PbChia1) from the clubroot pathogen Plasmodiophora brassicae and demonstrated that overexpressing it in the model plant Arabidopsis thaliana improves resistance to multiple pathogens.
Virulence · 9 authors, 4 centres
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The study isolated a chitinase gene (PbChia1) from the clubroot pathogen Plasmodiophora brassicae and demonstrated that overexpressing it in the model plant Arabidopsis thaliana improves resistance to multiple pathogens.
Researchers identified the chitinase PbChia1 from the clubroot pathogen P. brassicae and showed it binds and hydrolyzes chitin in vitro. They generated transgenic Arabidopsis plants overexpressing PbChia1 and challenged them with various pathogens. Overexpression significantly reduced P. brassicae resting spores and clubroot severity, with a 61.29% biocontrol effect, and increased plant survival and seed yield. The transgenic plants exhibited enhanced PAMP-triggered immune responses, including increased reactive oxygen species burst, MAPK activation, and expression of immune-related genes. Furthermore, PbChia1 overexpression conferred resistance to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and the necrotrophic fungi Sclerotinia sclerotiorum and Rhizoctonia solani. The findings suggest PbChia1 is a candidate gene for breeding broad-spectrum disease-resistant crops.