Photoprotection Against UV-Induced Skin Damage Using Hyaluronic Acid Produced by Lactiplantibacillus plantarum and Enterococcus durans

Current Microbiology · 5 authors, 2 centres

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TL;DR

This laboratory study isolated two probiotic bacteria, Lactiplantibacillus plantarum St3 and Enterococcus durans K11, that produce hyaluronic acid (HA). The HA produced showed antioxidant activity and protected human dermal keratinocytes from UVB radiation damage in vitro.

Full summary

433 CHARS

The study isolated 18 probiotic bacterial strains from various sources and screened them for hyaluronic acid (HA) production on skimmed milk medium. HA concentration was measured using a CTAB turbidimetric assay and confirmed by HPLC and FTIR analysis. The highest HA producers were identified as Lactiplantibacillus plantarum St3 and Enterococcus durans K11. The study is the first to report HA production by these specific strains.

STUDY TYPE

other

SPECIALTY

dermatology

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100% · A

ORIGINAL PAPER

PMC10299924

Photoprotection Against UV-Induced Skin Damage Using Hyaluronic Acid Produced by Lactiplantibacillus plantarum and Enterococcus durans

Current Microbiology

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ABSTRACT

Exposure to ultraviolet (UV) radiation is one of the major factors that causes skin aging, erythema, sunburns, and skin cancer. This study aimed to select probiotic bacterial isolates able to produce high yield of hyaluronic acid (HA) to be employed for skin photoprotection and other possible biological applications. The selected isolates K11 and St3 were able to produce the highest yields of HA 4.8 and 4.4 mg/ml, respectively. Both isolates were identified as Enterococcus durans strain K11 and Lactiplantibacillus plantarum strain St3 using 16S rRNA gene sequencing. The antioxidant activity of HA produced by E. durans strain K11 and L. plantarum strain St3 was (65.4 0.2%) and (66.6 0.1%), respectively. The viability of UVB-irradiated keratinocytes pre-treated with HA produced by E. durans strain K11 and L. plantarum strain St3 was 91.3 and 91.4%, respectively, compared with the control. While the viability of UVB-irradiated keratinocytes post-treated with HA produced by E. durans strain K11 and L. plantarum strain St3 was 86 and 88.5%, respectively. To the best of our knowledge, this is the first recordation of HA production by Enterococcus durans and Lactiplantibacillus plantarum which revealed a significant radioprotection of the human keratinocytes against UVB radiation . Supplementary Information The online version contains supplementary material available at 10.1007/s00284-023-03377-y.

CITATION EXPORT

Amany E. Shaheen, Hassan M. Gebreel, Loutfy A. Moussa et al.. Photoprotection Against UV-Induced Skin Damage Using Hyaluronic Acid Produced by Lactiplantibacillus plantarum and Enterococcus durans. Current Microbiology. (2023). https://doi.org/10.1007/s00284-023-03377-y

AUTHORS

5 · 2 CENTRES

Amany E. Shaheen, Hassan M. Gebreel, Loutfy A. Moussa, Abeer E. Zakaria, Waleed A. Nemr

1grid.429648.50000 0000 9052 0245Department of Radiation Microbiology, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
2grid.7269.a0000 0004 0621 1570Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt