International Journal of Molecular Sciences · 14 authors, 5 centres
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This exploratory study analyzed the lipid and metabolite cargo of platelet-derived extracellular vesicles (PL-EVs) from human subjects with mild and severe allergic inflammation versus controls. The central result is that PL-EVs exhibit a differential metabolic profile according to inflammation severity, with specific metabolites like ceramides and L-carnitine being more abundant in PL-EVs from mildly allergic patients compared to severe cases.
The study isolated and characterized small platelet-derived extracellular vesicles (PL-EVs) from platelet-rich plasma of control subjects and patients with mild or severe allergic inflammation using ultracentrifugation and size exclusion chromatography. Multiplatform metabolomics analysis revealed that PL-EVs from allergic patients contained distinct lipid and metabolite profiles compared to controls, with clearer separation between severe and control groups. Key findings include higher levels of ceramide (d18:1/24:0), specific triglycerides, and L-carnitine in PL-EVs from mildly allergic patients, which are metabolites involved in apoptosis and regulatory T-cell function. In contrast, PL-EVs from severely allergic patients showed dysregulation of metabolites related to arachidonic acid metabolism.
