Preliminary in vitro assessment of probiotic properties of Bacillus subtilis GM1, a spore forming bacteria isolated from goat milk

Iranian Journal of Veterinary Research · 3 authors, 2 centres

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TL;DR

This in vitro study isolated a Bacillus subtilis strain from goat milk and evaluated its potential as a probiotic. The strain showed tolerance to simulated gastrointestinal conditions and adhered to human intestinal cells, suggesting it could be a candidate for probiotic development.

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Researchers isolated bacterial strains from 40 goat milk samples, identifying one as Bacillus subtilis GM1. They conducted a series of in vitro tests to assess its probiotic potential. It was also negative for enterotoxin genes and susceptible to several antibiotics, indicating safety aspects. The study is limited by its preclinical, in vitro design, which cannot predict efficacy in a complex human gut environment or confirm safety in vivo. The findings suggest the strain has probiotic characteristics, but further in vivo and human studies are required.

STUDY TYPE

other

SPECIALTY

preclinical research

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AI pipeline

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100% · A

ORIGINAL PAPER

PMC10291520

Preliminary in vitro assessment of probiotic properties of Bacillus subtilis GM1, a spore forming bacteria isolated from goat milk

Iranian Journal of Veterinary Research

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ABSTRACT

Background: Species of the Bacillus genus have a long history of use in biotechnology. Some Bacillus strains have recently been identified for food applications and industrial as safe bacteria, which mostly have been recognized as probiotic strains. Aims: The primary purpose of the current study was to evaluate the probiotic characteristics of Bacillus subtilis strains isolated and identified from the goat milk samples. Methods: After sampling from 40 goat milk and cultivation, suspected colonies were subjected to biochemical and molecular identification. Then, the confirmed isolate was assessed for in vitro probiotic tests, including hemolysis and lecithinase properties, bile salt, acid, and artificial gastric juice resistance, antioxidant activity, antibiotics susceptibility, enterotoxin genes detection, and attachment capacity to the HT-29 cells. Results: Among 11 suspected isolates evaluated, only one isolate was identified as B. subtilis . In vitro tests for this strain showed similar results to other probiotic strains. The B. subtilis strain was susceptible to various antibiotics. The enterotoxin genes were not detected based on PCR assay. Concerning its probiotic characteristics assessment, especially tolerance to bile salts and acidic conditions, the Bacillus strain could have the potential to consider as a probiotic. Conclusion: Goat milk can be recommended as a source of Bacillus isolates. Also, the isolated strain showed high adaptability to the gastrointestinal environment, relatively equal percentages of adhesion properties, and some safety aspects, having the potential to be considered as an appropriate probiotic.

CITATION EXPORT

R. Daneshazari, M. Rabbani Khorasgani, A. Hosseini-Abari. Preliminary in vitro assessment of probiotic properties of Bacillus subtilis GM1, a spore forming bacteria isolated from goat milk. Iranian Journal of Veterinary Research. (2023). https://doi.org/10.22099/IJVR.2023.43891.6441

AUTHORS

3 · 2 CENTRES

R. Daneshazari, M. Rabbani Khorasgani, A. Hosseini-Abari

1Ph.D. Student in Microbiology, Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran;
2Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran