Probiotic powder ameliorates colorectal cancer by regulating Bifidobacterium animalis , Clostridium cocleatum , and immune cell composition

PLOS ONE · 15 authors, 8 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

This study used a mouse model of colorectal cancer to test a new probiotic powder.

Full summary

460 CHARS

This preclinical study evaluated a new probiotic powder in a mouse model of colorectal cancer (CRC). The researchers assessed the powder's effect on mouse survival, tumor size, intestinal barrier integrity, gut microbiota composition via 16S rDNA sequencing, immune cell populations via flow cytometry, and apoptotic protein expression via western blot. Limitations include the need for further study on specific mechanisms and potential combination therapies.

STUDY TYPE

other

SPECIALTY

oncology

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10010516

Probiotic powder ameliorates colorectal cancer by regulating Bifidobacterium animalis , Clostridium cocleatum , and immune cell composition

PLOS ONE

READ THE FULL PAPER

ABSTRACT

Abstract Based on the relationship between the gut microbiota and colorectal cancer, we developed a new probiotic powder for treatment of colorectal cancer. Initially, we evaluated the effect of the probiotic powder on CRC using hematoxylin and eosin staining, and evaluated mouse survival rate and tumor size. We then investigated the effects of the probiotic powder on the gut microbiota, immune cells, and apoptotic proteins using 16S rDNA sequencing, flow cytometry, and western blot, respectively. The results showed that the probiotic powder improved the intestinal barrier integrity, survival rate, and reduced tumor size in CRC mice. This effect was associated with changes in the gut microbiota. Specifically, the probiotic powder increased the abundance of Bifidobacterium animalis and reduced the abundance of Clostridium cocleatum . In addition, the probiotic powder resulted in decreased numbers of CD4 + Foxp3 + Treg cells, increased numbers of IFN-γ + CD8 + T cells and CD4 + IL-4 + Th2 cells, decreased expression of the TIGIT in CD4 + IL-4 + Th2 cells, and increased numbers of CD19 + GL-7 + B cells. Furthermore, the expression of the pro-apoptotic protein BAX was significantly increased in tumor tissues in response to the probiotic powder. In summary, the probiotic powder ameliorated CRC by regulating the gut microbiota, reducing Treg cell abundance, promoting the number of IFN-γ + CD8 + T cells, increasing Th2 cell abundance, inhibiting the expression of TIGIT in Th2 cells, and increasing B cell abundance in the immune microenvironment of CRC, thereby increasing the expression of BAX in CRC.

CITATION EXPORT

Xiaojuan Yang, Qian Cao, Bin Ma et al.. Probiotic powder ameliorates colorectal cancer by regulating Bifidobacterium animalis, Clostridium cocleatum, and immune cell composition. PLOS ONE. (2023). https://doi.org/10.1371/journal.pone.0277155

AUTHORS

15 · 8 CENTRES

Xiaojuan Yang, Qian Cao, Bin Ma, Yuhan Xia, Miao Liu, Jinhua Tian, Jian Chen, Chunxia Su, Xiangguo Duan, Hiroyasu Nakano, Hiroyasu Nakano, Hiroyasu Nakano, Hiroyasu Nakano, Hiroyasu Nakano, Hiroyasu Nakano

1School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, China
2College of Clinical Medicine, Ningxia Medical University, Yinchuan, China
3Department of Oncology Surgery, The First People’s Hospital of Yinchuan, Yinchuan, China
4Department of Nutrition, General Hospital of Ningxia Medical University, Yinchuan, China
5Guolong Hospital, Yinchuan, China
6Department of Pathogen Biology and Immunology, School of Basic Medical Science, Ningxia Medical University, Yinchuan, China
7Department of Laboratory Surgery, General Hospital of Ningxia Medical University, Yinchuan, China
8Toho University Graduate School of Medicine, JAPAN