Psoralen synergizes with exosome-loaded SPC25 to alleviate senescence of nucleus pulposus cells in intervertebral disc degeneration

Journal of Orthopaedic Surgery and Research · 7 authors, 1 centre

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

NP cells were then treated with psoralen, exosomes from adipose-derived stem cells overexpressing SPC25 (ADSCs-oe-SPC25-Exos), or a combination of both.

Full summary

785 CHARS

NP cells were then treated with psoralen, exosomes from adipose-derived stem cells overexpressing SPC25 (ADSCs-oe-SPC25-Exos), or a combination of both. Both individual treatments promoted cell proliferation and expression of aggrecan, COL2A1, Bcl-2, and CDK2, while reducing Bax, p16, p21 expression, and inflammatory factors, thereby inhibiting senescence, cycle arrest, and apoptosis. Notably, the combined treatment further decelerated NP cell senescence and cycle arrest compared to either agent alone, indicating a synergistic effect. The authors acknowledge that experiments were limited to the cellular level and that clinical sample numbers were limited, with animal experiments needed to confirm findings. The study has not yet been validated in vivo or in clinical settings.

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10594823

Psoralen synergizes with exosome-loaded SPC25 to alleviate senescence of nucleus pulposus cells in intervertebral disc degeneration

Journal of Orthopaedic Surgery and Research

READ THE FULL PAPER

ABSTRACT

Abstract Objective To explore the mechanism of psoralen synergized with exosomes (exos)-loaded SPC25 on nucleus pulposus (NP) cell senescence in intervertebral disc degeneration (IVDD). Methods IVDD cellular models were established on NP cells by tert -butyl hydroperoxide (TBHP) induction, followed by the treatment of psoralen or/and exos from adipose-derived stem cells (ADSCs) transfected with SPC25 overexpression vector (ADSCs-oe-SPC25-Exos). The viability, cell cycle, apoptosis, and senescence of NP cells were examined, accompanied by the expression measurement of aggrecan, COL2A1, Bcl-2, Bax, CDK2, p16, and p21. Results After TBHP-induced NP cells were treated with psoralen or ADSCs-oe-SPC25-Exos, cell proliferation and the expression of aggrecan, COL2A1, Bcl-2, and CDK2 were promoted; however, the expression of Bax, p16, p21, and inflammatory factors was decreased, and cell senescence, cycle arrest, and apoptosis were inhibited. Of note, psoralen combined with ADSCs-oe-SPC25-Exos further decelerated NP cell senescence and cycle arrest compared to psoralen or ADSCs-oe-SPC25-Exos alone. Conclusion Combined treatment of psoralen and ADSCs-oe-SPC25-Exos exerted an alleviating effect on NP cell senescence, which may provide an insightful idea for IVDD treatment. Supplementary Information The online version contains supplementary material available at 10.1186/s13018-023-04085-w. Keywords: Psoralen, Exosomes, SPC25, Nucleus pulposus, Senescence, Intervertebral disc degeneration

CITATION EXPORT

Lei Yang, Zhaoyong Li, Chao Zhang et al.. Psoralen synergizes with exosome-loaded SPC25 to alleviate senescence of nucleus pulposus cells in intervertebral disc degeneration. Journal of Orthopaedic Surgery and Research. (2023). https://doi.org/10.1186/s13018-023-04085-w

AUTHORS

7 · 1 CENTRES

Lei Yang, Zhaoyong Li, Chao Zhang, Shuofu Li, Long Chen, Shaofeng Yang, Yantao Guo

1Department of Spine, The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, No. 95 Shaoshan Middle Road, Yuhua District, Changsha, 410007 Hunan People’s Republic of China