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TL;DR
Filter paper eluates retained comparable neutralising antibody responses to paired sera after storage on filter paper for 2–28 days at room temperature, supporting DBS as a viable collection method for SARS-CoV-2 serology.
Scientific Reports · 13 authors, 61 centres
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Filter paper eluates retained comparable neutralising antibody responses to paired sera after storage on filter paper for 2–28 days at room temperature, supporting DBS as a viable collection method for SARS-CoV-2 serology.
This study evaluated whether filter paper storage affects quantification of neutralising antibody (nAb) responses against SARS-CoV-2 using pseudovirus particle (PVP) assays. VDBS eluates of 50 μL showed the highest level of agreement to paired sera, while 15 μL VDBS overestimated nAb responses due to excess filter paper absorbing elution buffer. FDBS eluates retained comparable nAb responses to paired sera after storage on filter paper for 2–28 days at room temperature (approximately 20 °C). However, FDBS showed decreased sensitivity (73–88%) for IC values, likely attributable to unknown blood volume collected. The study supports the use of DBS for SARS-CoV-2 serology, noting that DBS are less invasive, low cost, and stable at ambient temperatures. Limitations include the UK-specific room temperature and the need for further exploration of nAb stability at higher ambient temperatures.