Rapid Pathogen Purge by Photosensitive Arginine–Riboflavin Carbon Dots without Toxicity

Materials · 4 authors, 3 centres

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AGENT 01 · STRUCTURED SUMMARY

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TL;DR

Researchers synthesized arginine-based carbon dots (CDs) and modified them with riboflavin to create a photosensitive nanomaterial. The modified CDs showed rapid, total eradication of common bacteria when exposed to UV-A light for 30 minutes, while maintaining low cytotoxicity and hemocompatibility in laboratory tests.

Full summary

1,010 CHARS

This preclinical study synthesized nitrogen-doped arginine carbon dots (Arg CDs) via microwave carbonization and modified them with the fluorescent dyes fluorescein sodium salt (Arg-FSS) and riboflavin (Arg-RBF) to enhance photodynamic properties. The Arg-RBF CDs exhibited dual fluorescence, with a green emission at 520 nm. In vitro cytotoxicity testing on L929 fibroblasts showed the Arg-RBF CDs had 91.2 ± 0.7% cell viability at 1000 μg/mL. Hemocompatibility assays indicated hemolysis ratios below 5% and no significant blood clotting effects for all CD types at 1000 μg/mL. The CDs demonstrated broad-spectrum antimicrobial activity against bacteria and fungi, with minimum inhibitory concentrations (MICs) reported. The Arg-RBF CDs showed faster and more effective antibacterial activity, with total bacterial eradication upon 30 minutes of UV-A light exposure. The findings suggest Arg-RBF CDs are a promising, biocompatible candidate for photodynamic antimicrobial therapy, pending further evaluation.

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10573186

Rapid Pathogen Purge by Photosensitive Arginine–Riboflavin Carbon Dots without Toxicity

Materials

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ABSTRACT

Photo-activatable antipathogenic carbon dots (CDs) were prepared by carbonization of citric acid and arginine (Arg) via 3 min microwave treatment for use in the eradication of common microorganisms. Nitrogen-doped Arg CDs were spherical in shape with a size range of 0.5 to 5 nm. The Arg CDs were modified with fluorescent dyes, such as fluorescein sodium salt (FSS, as Arg-FSS) and riboflavin (RBF, as Arg-RBF), to improve antimicrobial potency by enhancing their application in photodynamic therapy. The modified Arg CDs afforded fluorescence emission properties at 520 nm in the green region in addition to excellent blue fluorescence intensity at 420 nm under 345 nm excitation upon their FSS and RBF conjugation, respectively. Although the cytotoxicity of Arg CDs was decreased for Arg-RBF CDs to 91.2 ± 0.7% cell viability for fibroblasts, the Arg-based CDs could be safely used for intravenous applications at 1000 μg/mL concentration. The Arg CDs showed broad-spectrum antimicrobial activity against common pathogens and the minimum inhibitory concentration of Arg CDs was almost two-fold decreased for the modified forms without UV light. However, faster and more effective antibacterial activity was determined for photosensitive Arg-RBF CDs, with total bacterial eradication upon UV-A light exposure for 30 min.

CITATION EXPORT

Selin S. Suner, Venkat R. Bhethanabotla, Ramesh S. Ayyala et al.. Rapid Pathogen Purge by Photosensitive Arginine–Riboflavin Carbon Dots without Toxicity. Materials. (2023). https://doi.org/10.3390/ma16196512

AUTHORS

4 · 3 CENTRES

Selin S. Suner, Venkat R. Bhethanabotla, Ramesh S. Ayyala, Nurettin Sahiner

1Department of Chemistry, Faculty of Sciences & Arts, and Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University Terzioglu Campus, Canakkale 17100, Turkey; [email protected]
2Department of Chemical, Biological, and Materials Engineering, Materials Science and Engineering Program, University of South Florida, Tampa, FL 33620, USA; [email protected]
3Department of Ophthalmology, Morsani College of Medicine, University of South Florida Eye Institute,12901 Bruce B Down Blvd, MDC 21, Tampa, FL 33612, USA; [email protected]

EDITOR / REVIEWER

Junkai Ren