Recombinase Polymerase Amplification Assay with Lateral Flow Strips for Rapid Detection of Candidiasis Due to Candida parapsilosis

Current Microbiology · 6 authors, 3 centres

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TL;DR

The assay demonstrated high sensitivity and specificity, with results available in under 40 minutes without requiring expensive instruments.

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It was compared to quantitative PCR (qPCR) and showed comparable results when testing clinical samples. Limitations include the risk of aerosol contamination in laboratory settings, which the authors suggest can be mitigated through home testing. The implications are for the development of a rapid, portable, and user-friendly diagnostic tool for Candida parapsilosis, particularly useful in settings without sophisticated laboratory facilities.

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other

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infectious disease

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AI pipeline

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ORIGINAL PAPER

PMC10195124

Recombinase Polymerase Amplification Assay with Lateral Flow Strips for Rapid Detection of Candidiasis Due to Candida parapsilosis

Current Microbiology

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ABSTRACT

Candida parapsilosis is a common cause of candidiasis among hospitalized patients, often surpassing Candida albicans . Due to the recent increase in C. parapsilosis infections, there is an urgent need for rapid, sensitive, and real-time on-site detection of nucleic acids for timely diagnosis of candidiasis. We developed an assay for detection of C. parapsilosis by combining recombinase polymerase amplification (RPA) with a lateral flow strip (LFS). The RPA-LFS assay was used to amplify the beta-1,3-glucan synthase catalytic subunit 2 ( FKS2 ) gene of C. parapsilosis with a primer–probe set optimized by introducing base mismatches (four bases modified by the probe and one by the reverse primer) to achieve specific and sensitive detection of clinical samples. The RPA assays can rapidly amplify and visualize a target gene within 30 min, while the entire process can be completed within 40 min by pre-processing the sample. The product of RPA has two chemical labels, FITC and Biotin, of the amplification product can be carefully on the strip. The sensitivity and specificity of the RPA-LFS assay were determined by analysis of 35 common clinical pathogens and 281 clinical samples against quantitative PCR. The results confirmed that the proposed RPA-LFS assay is a reliable molecular diagnostic method for the detection of C. parapsilosis to meet the urgent need for rapid, specific, sensitive, and portable field testing. Supplementary Information The online version contains supplementary material available at 10.1007/s00284-023-03318-9.

CITATION EXPORT

Bo Zhu, Lei Wang, Yingzhi Lu et al.. Recombinase Polymerase Amplification Assay with Lateral Flow Strips for Rapid Detection of Candidiasis Due to Candida parapsilosis. Current Microbiology. (2023). https://doi.org/10.1007/s00284-023-03318-9

AUTHORS

6 · 3 CENTRES

Bo Zhu, Lei Wang, Yingzhi Lu, Cheng Chen, Kun Wang, Lei Zhang

1grid.452247.2Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, China
2grid.460072.7Central Laboratory of Hospital, The First People’s Hospital of Lianyungang, Xuzhou Medical University Affiliated Hospital, Lianyungang, China
3Department of Oncology & Department of Laboratory Medicine, The Second People’s Hospital of Lianyungang (Cancer Hospital of Lianyungang), Lianyungang, China