Response surface methodology based optimization of keratinase from Bacillus velezensis strain ZBE1 and nanoparticle synthesis, biological and molecular characterization

Saudi Journal of Biological Sciences · 5 authors, 3 centres

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TL;DR

A keratinolytic Bacillus velezensis strain ZBE1 was isolated from a deep forest region and assessed for keratinase production using chicken feather meal as substrate.

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A keratinolytic Bacillus velezensis strain ZBE1 was isolated from a deep forest region and assessed for keratinase production using chicken feather meal as substrate. Implications are confined to potential industrial application for keratinous waste management.

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other

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preclinical research

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ORIGINAL PAPER

PMC10495650

Response surface methodology based optimization of keratinase from Bacillus velezensis strain ZBE1 and nanoparticle synthesis, biological and molecular characterization

Saudi Journal of Biological Sciences

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ABSTRACT

Abstract The increasing demands of keratinases for biodegradation of recalcitrant keratinaceous waste like chicken feathers has lead to research on newer potential bacterial keratinases to produce high-value products with biological activities. The present study reports a novel keratinolytic bacterium Bacillus velezensis strain ZBE1 isolated from deep forest soil of Western Ghats of Karnataka, which possessed efficient feather keratin degradation capability and induced keratinase production. Production kinetics depicts maximum keratinase production (11.65 U/mL) on 4th day with protein concentration of 0.61 mg/mL. Effect of various physico-chemical factors such as, inoculum size, metal ions, carbon and nitrogen sources, pH and temperature influencing keratinase production were optimized and 3.74 folds enhancement was evidenced through response surface methodology. Silver (AgNP) and zinc oxide (ZnONP) nanoparticles with keratin hydrolysate produced from chicken feathers by the action of keratinase were synthesized and verified with UV–Visible spectroscopy that revealed biological activities like, antibacterial action against Bacillus cereus and Escherichia coli . AgNP and ZnONP also showed potential antioxidant activities through radical scavenging activities by ABTS and DPPH. AgNP and ZnONP revealed cytotoxic effect against MCF-7 breast cancer cell lines with IC 50 of 5.47 µg/ml and 62.26 µg/ml respectively. Characterizations of nanoparticles were carried out by Fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray, X-ray diffraction, thermogravimetric analysis and atomic force microscopy analysis to elucidate the thermostability, structure and surface attributes. The study suggests the prospective applications of keratinase to trigger the production of bioactive value-added products and significant application in nanotechnology in biomedicine. Keywords: Bacillus velezensis , Keratinase, Response surface methodology, Nanoparticles, Analytical characterization, Biological properties

CITATION EXPORT

Archana G Revankar, Zabin K Bagewadi, Neha P Bochageri et al.. Response surface methodology based optimization of keratinase from Bacillus velezensis strain ZBE1 and nanoparticle synthesis, biological and molecular characterization. Saudi Journal of Biological Sciences. (2023). https://doi.org/10.1016/j.sjbs.2023.103787

AUTHORS

5 · 3 CENTRES

Archana G Revankar, Zabin K Bagewadi, Neha P Bochageri, TM Yunus Khan, Shaik Mohamed Shamsudeen

aDepartment of Biotechnology, KLE Technological University, Hubballi, Karnataka 580031, India
bDepartment of Mechanical Engineering, College of Engineering, King Khalid University, Abha 61421, Saudi Arabia
cDepartment of Diagnostic dental science and Oral Biology, College of Dentistry, King Khalid University, Abha 61421, Saudi Arabia

Archana G Revankar, Zabin K Bagewadi, Neha P Bochageri et al.. Response surface methodology based optimization of keratinase from Bacillus velezensis strain ZBE1 and nanoparticle synthesis, biological and molecular characterization. Saudi Journal of Biological Sciences. (2023). https://doi.org/10.1016/j.sjbs.2023.103787