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other·ophthalmology, cornea, preclinical research, biomedical research·PMC10526416

ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing

Biomolecules · 5 authors, 1 centre

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TL;DR

This preclinical study used in vitro and in vivo models to show that reactive oxygen species fragment high-molecular-weight hyaluronan, and that the resulting oxidized fragments have different effects on corneal epithelial cell proliferation, migration, and wound healing compared to native hyaluronan of the same molecular weight.

Full summary

577 CHARS

The study investigated whether hyaluronan (HA) fragments generated by oxidation with H2O2 or HOCl have distinct physiological properties compared to native HA of the same molecular weight. In vitro, oxidized HA produced by HOCl inhibited human corneal epithelial cell proliferation and migration differently than size-matched native low-molecular-weight HA. The authors conclude that both the reduction in molecular weight and the chemical modifications from oxidation alter HA's functions, which is relevant for HA-based therapies in conditions with elevated oxidative stress.

STUDY TYPE

other

SPECIALTY

ophthalmology

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AI pipeline

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100% · A

ORIGINAL PAPER

PMC10526416

ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing

Biomolecules

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ABSTRACT

Abstract A buildup of reactive oxygen species (ROS) occurs in virtually all pathological conditions. Hyaluronan (HA) is a major extracellular matrix component and is susceptible to oxidation by reactive oxygen species (ROS), yet the precise chemical structures of oxidized HA products (oxHA) and their physiological properties remain largely unknown. This study characterized the molecular weight (MW), structures, and physiological properties of oxHA. For this, high-molecular-weight HA (HMWHA) was oxidized using increasing molar ratios of hydrogen peroxide (H 2 O 2 ) or hypochlorous acid (HOCl). ROS lead to the fragmentation of HA, with the oxHA products produced by HOCl exhibiting an altered chemical structure while those produced by H 2 O 2 do not. HMWHA promotes the viability of human corneal epithelial cells (hTCEpi), while low MWHA (LMWHA), ultra-LMWHA (ULMWHA), and most forms of oxHA do not. HMWHA and LMWHA promote hTCEpi proliferation, while ULMWHA and all forms of oxHA do not. LMWHA and some forms of oxHA promote hTCEpi migration, while HMWHA does not. Finally, all native forms of HA and oxHA produced by HOCl promote in vivo corneal wound healing, while oxHA produced by H 2 O 2 does not. Taken together, our results show that HA fragmentation by ROS can alter the physiological activity of HA by altering its MW and structure. Keywords: high-molecular-weight hyaluronan, low-molecular-weight hyaluronan, oxidative stress, wound healing, corneal epithelium

CITATION EXPORT

Xiao Lin, Isabel Y Moreno, Lawrence Nguyen et al.. ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing. Biomolecules. (2023). https://doi.org/10.3390/biom13091385

AUTHORS

5 · 1 CENTRES

Xiao Lin, Isabel Y Moreno, Lawrence Nguyen, Tarsis F Gesteira, Vivien J Coulson-Thomas

1College of Optometry, University of Houston, 4401 Martin Luther King Boulevard, Houston, TX 77204-2020, USA; [email protected] (X.L.); [email protected] (I.Y.M.); [email protected] (L.N.); [email protected] (T.F.G.)