International Journal of Molecular Sciences · 3 authors, 5 centres
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This in vitro study used conditioned medium from calcium-sensing receptor (CaSR)-activated SW872 pre-adipocytes to treat HepG2 liver cells. The central result was that this conditioned medium induced cellular senescence, mitochondrial dysfunction, and an inflammatory response in the HepG2 cells.
The study used an in vitro model to investigate how secreted factors from CaSR-activated SW872 pre-adipocytes affect HepG2 hepatocyte-like cells. HepG2 cells treated with CMcin showed increased senescence markers (SA-β-GAL, p21), pro-inflammatory cytokines (IL-1β, TNF-α, CCL2), and mitochondrial dysfunction (decreased membrane potential, increased fragmentation, altered protein expression like MFN2, DRP1, PGC-1α). Blocking IL-1β or TNF-α in the CM prevented some senescence effects, and inhibiting mitochondrial fragmentation with Mdivi-1 reduced cytokine expression. The study demonstrates that CaSR activation in pre-adipocytes can promote a senescent and inflammatory phenotype in hepatic cells via secreted factors, with mitochondrial fragmentation playing a role.
