Antioxidants · 6 authors, 2 centres
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This in vitro study synthesized Ganoderma lucidum spore oil-functionalized selenium nanoparticles (GLSO@SeNPs) and tested them on INS-1E rat insulinoma β-cells exposed to palmitic acid. GLSO@SeNPs significantly inhibited palmitic acid-induced apoptosis by enhancing antioxidant enzyme activity, reducing ROS levels, and modulating the MAPK signaling pathway.
INS-1E rat insulinoma β-cells were pretreated with GLSO@SeNPs, SeNPs, or GLSO@NEs and then exposed to 0.3 mM palmitic acid for 24 hours to model lipotoxicity. GLSO@SeNPs exhibited good stability and biocompatibility, inhibited PA-induced apoptosis in a concentration-dependent manner, and reduced G2/M phase arrest. GLSO@SeNPs reversed PA-induced mitochondrial membrane potential decline and morphological damage, significantly removed intracellular ROS, and enhanced activity of antioxidant enzymes including TrxR, SOD, CAT, GSH, and GPx while eliminating MDA. The composite material demonstrated more significant antioxidant activity than either component alone. Limitations include the exclusively in vitro design, with no in vivo animal experiments or pharmacokinetic studies conducted.
