SEARCHNARRATIVE_REVIEWinfectious disease, biomedical research

narrative_review·infectious disease, biomedical research, preclinical research·PMC10485009

Strategy to combat biofilms: a focus on biofilm dispersal enzymes

NPJ Biofilms and Microbiomes · 7 authors, 5 centres

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TL;DR

This narrative review summarizes enzymatic strategies, particularly glycoside hydrolases, proteases, and deoxyribonucleases, for dispersing bacterial biofilms.

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This narrative review examines enzymatic strategies to combat bacterial biofilms, focusing on dispersal mechanisms of glycoside hydrolases, proteases, and deoxyribonucleases. Central results highlight that enzymes can inhibit biofilm formation, disperse established biofilms, and synergize with antibiotics. The authors note that while in vitro results are promising, only a few in vivo studies and clinical trials have been conducted. Key limitations include unresolved issues of enzyme toxicity, stability, and compatibility. The implications are that further development is needed to create safe and potent enzymes for clinical use.

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narrative_review

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infectious disease

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ORIGINAL PAPER

PMC10485009

Strategy to combat biofilms: a focus on biofilm dispersal enzymes

NPJ Biofilms and Microbiomes

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ABSTRACT

Bacterial biofilms, which consist of three-dimensional extracellular polymeric substance (EPS), not only function as signaling networks, provide nutritional support, and facilitate surface adhesion, but also serve as a protective shield for the residing bacterial inhabitants against external stress, such as antibiotics, antimicrobials, and host immune responses. Biofilm-associated infections account for 65-80% of all human microbial infections that lead to serious mortality and morbidity. Tremendous effort has been spent to address the problem by developing biofilm-dispersing agents to discharge colonized microbial cells to a more vulnerable planktonic state. Here, we discuss the recent progress of enzymatic eradicating strategies against medical biofilms, with a focus on dispersal mechanisms. Particularly, we review three enzyme classes that have been extensively investigated, namely glycoside hydrolases, proteases, and deoxyribonucleases.

CITATION EXPORT

Shaochi Wang, Yanteng Zhao, Alexandra P. Breslawec et al.. Strategy to combat biofilms: a focus on biofilm dispersal enzymes. NPJ Biofilms and Microbiomes. (2023). https://doi.org/10.1038/s41522-023-00427-y

AUTHORS

7 · 5 CENTRES

Shaochi Wang, Yanteng Zhao, Alexandra P. Breslawec, Tingting Liang, Zhifen Deng, Laura L. Kuperman, Qiuning Yu

1https://ror.org/056swr059grid.412633.1Otorhinolaryngology Hospital, The First Affiliated Hospital of Zhengzhou University, 450052 Zhengzhou, China
2https://ror.org/056swr059grid.412633.1Translational Medicine Center, The First Affiliated Hospital of Zhengzhou University, 450052 Zhengzhou, China
3https://ror.org/047s2c258grid.164295.d0000 0001 0941 7177Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20740 USA
4https://ror.org/003xyzq10grid.256922.80000 0000 9139 560XKey Laboratory of Natural Medicine and Immune-Engineering of Henan Province, Henan University Jinming Campus, 475004 Kaifeng, Henan China
5Present Address: Mirimus Inc., 760 Parkside Avenue, Brooklyn, NY 11226 USA