other·preclinical research, biomedical research, endocrinology, animal science, research methods·PMC10135389
Studying and Analyzing Humane Endpoints in the Fructose-Fed and Streptozotocin-Injected Rat Model of Diabetes
Animals : an Open Access Journal from MDPI · 12 authors, 8 centres
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A humane endpoint scoring system with 14 welfare parameters was evaluated in Sprague-Dawley rats with fructose-fed and streptozotocin-induced type 2 diabetes over seven weeks. The scoring table was found suitable for monitoring animal welfare, with dehydration, grooming, posture, abdominal appearance, and fecal appearance identified as the most effective parameters, and glycemia was significantly higher in induced versus control animals.
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Male Sprague-Dawley rats were divided into control and induced groups. Induced animals received 10% fructose solution for 14 days followed by streptozotocin (40 mg/kg) intraperitoneally. Weekly assessments included body weight, water and food consumption, blood glucose at three time points, and a 14-parameter welfare score sheet. After seven weeks the rats were euthanized. Induced animals exhibited weight loss, polyuria, polyphagia, polydipsia, dehydration, lack of grooming, narrowed orbital area, curved posture, liquid or pasty diarrhea, and abdominal distension. None of the animals reached the critical humane endpoint score of four. The study had no animal deaths and validated both the diabetes model and the welfare scoring table.
