SUMOylation regulates Lem2 function in centromere clustering and silencing

Journal of Cell Science · 10 authors, 4 centres

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TL;DR

Suppressing Lem2 SUMOylation specifically is sufficient to rescue centromeric defects in nup132Δ cells.

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The authors found that centromere-related defects in nup132Δ cells, previously attributed to Pli1 destabilisation, are instead driven by enhanced SUMOylation at the nuclear periphery. Suppressing global or Lem2-specific SUMOylation rescued centromeric defects. Unexpectedly, hyper-SUMOylation showed opposing roles: detrimental to centromere silencing yet capable of partially rescuing clustering defects in csi1Δ cells. Statistical analysis used chi-squared tests for localization data.

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biomedical research

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ORIGINAL PAPER

PMC10730020

SUMOylation regulates Lem2 function in centromere clustering and silencing

Journal of Cell Science

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ABSTRACT

ABSTRACT Regulation by the small modifier SUMO is heavily dependent on spatial control of enzymes that mediate the attachment and removal of SUMO on substrate proteins. Here, we show that in the fission yeast Schizosaccharomyces pombe , delocalisation of the SUMO protease Ulp1 from the nuclear envelope results in centromeric defects that can be attributed to hyper-SUMOylation at the nuclear periphery. Unexpectedly, we find that although this localised hyper-SUMOylation impairs centromeric silencing, it can also enhance centromere clustering. Moreover, both effects are at least partially dependent on SUMOylation of the inner nuclear membrane protein Lem2. Lem2 has previously been implicated in diverse biological processes, including the promotion of both centromere clustering and silencing, but how these distinct activities are coordinated was unclear; our observations suggest a model whereby SUMOylation serves as a regulatory switch, modulating Lem2 interactions with competing partner proteins to balance its roles in alternative pathways. Our findings also reveal a previously unappreciated role for SUMOylation in promoting centromere clustering. Keywords: Lem2, SUMOylation, Ubiquitylation, Schizosaccharomyces pombe , Centromeres

CITATION EXPORT

Joanna Strachan, Orsolya Leidecker, Christos Spanos et al.. SUMOylation regulates Lem2 function in centromere clustering and silencing. Journal of Cell Science. (2023). https://doi.org/10.1242/jcs.260868

AUTHORS

10 · 4 CENTRES

Joanna Strachan, Orsolya Leidecker, Christos Spanos, Clementine Le Coz, Elliott Chapman, Ana Arsenijevic, Haidao Zhang, Ning Zhao, Steven H Spoel, Elizabeth H Bayne

1Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, UK
2Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Strasse 9b, Cologne 50931, Germany
3Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK
4Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK