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TL;DR
Using fungal molecular genetics and bioinformatic comparisons, the authors characterize the V. dahliae JR2 clock proteins Frq and Frh and their Frq–Frh complex.
Journal of Fungi · 8 authors, 1 centre
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Using fungal molecular genetics and bioinformatic comparisons, the authors characterize the V. dahliae JR2 clock proteins Frq and Frh and their Frq–Frh complex.
Using fungal molecular genetics and bioinformatic comparisons, the authors characterize the V. dahliae JR2 clock proteins Frq and Frh and their Frq–Frh complex. Both proteins localized predominantly to hyphal nuclei. A functional Frq–Frh complex was required for normal conidiation, because deletion of FRQ or introduction of an FRH^R806H^ point mutation reduced conidiation. FRQ deletion, FRH mutation, and WC1 deletion each increased microsclerotia formation in the light, while WC1 deletion reduced microsclerotia formation in darkness. The Frq–Frh complex thus light-dependently delays Sfl1-induced microsclerotia formation, whereas combined effects of Sfl1 and Frq slightly enhanced symptom induction in tomato. The work is preclinical, with no human endpoints, and limitations include the inability to generate a complete FRH deletion and species-specific differences versus Neurospora, Beauveria, and Metarhizium.