The Intestinal Microbiota Composition in Early and Late Stages of Diabetic Kidney Disease

Microbiology Spectrum · 6 authors, 4 centres

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TL;DR

This study used 16S rRNA sequencing to compare gut bacteria in people with diabetes alone, early diabetic kidney disease (DKD), and late DKD. It found that specific bacterial changes, particularly a decrease in Agathobacter, were most prominent in early DKD and could help distinguish disease stages.

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The study investigated gut microbiota composition in three groups: diabetes mellitus (DM), early DKD (DNa), and late DKD (DNb) using 16S rRNA gene sequencing of fecal samples. DNa. Prevotella_9 and Roseburia were significantly decreased in DNa and DNb compared to DM. Levels of Agathobacter, Prevotella_9, Lachnospira, and Roseburia were positively correlated with eGFR and negatively correlated with microalbuminuria, 24h urinary protein, and serum creatinine. The area under the curve for Agathobacter was 83.33% for distinguishing DM from DNa, and 83.60% for distinguishing DNa from DNb. The authors conclude that gut microbiota dysbiosis occurs in both early and late DKD, with changes most pronounced in early DKD, and propose Agathobacter as a potential biomarker. Limitations include small sample sizes, limited group divisions, and a cross-sectional design.

STUDY TYPE

cohort

SPECIALTY

nephrology

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AI pipeline

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ORIGINAL PAPER

PMC10434009

The Intestinal Microbiota Composition in Early and Late Stages of Diabetic Kidney Disease

Microbiology Spectrum

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ABSTRACT

ABSTRACT Many studies have suggested that gut microbiota dysbiosis may be one of the pathogenesis factors of diabetes mellitus (DM), while it is not clear whether it is involved in the development of diabetic kidney diseases (DKD). The objective of this study was to determine bacterial taxa biomarkers during the progression of DKD by investigating bacterial compositional changes in early and late DKD. 16S rRNA gene sequencing was performed on fecal samples, including the diabetes mellitus (DM), DNa (early DKD), and DNb (late DKD) groups. Taxonomic annotation of microbial composition was performed. Samples were sequenced on the Illumina NovaSeq platform. At the genus level, we found counts of Fusobacterium , Parabacteroides , and Ruminococcus_gnavus were significantly elevated both in the DNa group ( P = 0.0001, 0.0007, and 0.0174, respectively) and the DNb group ( P < 0.0001, 0.0012, and 0.0003, respectively) compared with those in the DM group. Only the level of Agathobacter was significantly decreased in the DNa group than the DM group and in the DNb group than the DNa group. Counts of Prevotella_9 , Roseburia were significantly decreased in the DNa group compared with those in the DM group ( P = 0.001 and 0.006, respectively) and in the DNb group compared with those in the DM group ( P < 0.0001 and 0.003, respectively). Levels of Agathobacter, Prevotella_9, Lachnospira , and Roseburia were positively correlated with an estimated glomerular filtration rate (eGFR), but negatively correlated with microalbuminuria (MAU), 24 h urinary protein quantity (24hUP), and serum creatinine (Scr). Moreover, the areas under the curve (AUCs) of Agathobacter and Fusobacteria were 83.33% and 80.77%, respectively, for the DM and DNa cohorts, respectively. Notably, the largest AUC for DNa and DNb cohorts was also that of Agathobacter at 83.60%. Gut microbiota dysbiosis was found in the early and late stages of DKD, especially in the early stage. Agathobacter may be the most promising intestinal bacteria biomarker that can help distinguish different stages of DKD. IMPORTANCE It is not clear as to whether gut microbiota dysbiosis is involved in the progression of DKD. This study may be the first to explore gut microbiota compositional changes in diabetes, early-DKD, and late DKD. We identify different gut microbial characteristics during different stages of DKD. Gut microbiota dysbiosis is found in the early and late stages of DKD. Agathobacter may be the most promising intestinal bacteria biomarker that can help distinguish different stages of DKD, although further studies are warranted to illustrate these mechanisms.

CITATION EXPORT

Li Zhang, Qi-Yu Lu, Hao Wu et al.. The Intestinal Microbiota Composition in Early and Late Stages of Diabetic Kidney Disease. Microbiology Spectrum. (2023). https://doi.org/10.1128/spectrum.00382-23

AUTHORS

6 · 4 CENTRES

Li Zhang, Qi-Yu Lu, Hao Wu, Yan-Li Cheng, Jing Kang, Zhong-Gao Xu

a Department of Nephrology, The First Hospital of Jilin University, Changchun, Jilin Province, China
b Department of Thyroid Surgery, General surgery center, The First Hospital of Jilin University, Changchun, Jilin Province, China
c Department of Endocrinology, The Second Hospital of Jilin University, Changchun, Jilin Province, China
Universita degli Studi di Parma

EDITOR / REVIEWER

Francesca Turroni