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TL;DR
This study used transgenic apple lines to show that the m6A reader protein MhYTP2 enhances tolerance to low nitrogen by stabilizing mRNAs involved in nitrogen catabolism, root development, and autophagy.
Horticulture Research · 7 authors, 7 centres
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This study used transgenic apple lines to show that the m6A reader protein MhYTP2 enhances tolerance to low nitrogen by stabilizing mRNAs involved in nitrogen catabolism, root development, and autophagy.
This preclinical study investigated the function of the apple m6A reader protein MhYTP2 in response to low nitrogen (N) stress. Researchers used transgenic apple lines overexpressing MhYTP2 and performed RNA immunoprecipitation-seq and electrophoretic mobility shift assays to identify direct mRNA targets. They found MhYTP2 binds to and stabilizes the mRNAs of MdALN, MdPIDL, MdTTG1, and MdATG8A, while accelerating the degradation of MdRHD3 mRNA. Overexpression of MhYTP2 enhanced plant resistance to low N, as shown by increased allantoinase activity, improved root growth, and enhanced autophagic activity. Knockdown or overexpression of these specific target genes in MhYTP2-overexpressing plants abolished the enhanced low N tolerance. The implications suggest a post-transcriptional mechanism for N starvation tolerance in plants.