Journal of Neurogastroenterology and Motility · 9 authors, 2 centres
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This pilot open-label study measured probiotic fecal detection and whole gut transit time in 30 healthy adults receiving a 5-strain probiotic for 2 weeks. Most strains persisted 3–6 days after cessation, but Bifidobacterium longum R0175 persisted significantly longer (15–30 days) in a subset of participants with intermediate transit times and a distinct microbiota composition.
Thirty healthy adults received a five-strain probiotic (30 × 10^9 CFU/day) for 2 weeks, flanked by 4-week washout periods, with 18 stool collections analyzed by qPCR for strain-specific detection. Machine learning classified WGTT subgroups based on differentially abundant taxa with high accuracy. Probiotic strains were detected in feces approximately 1–2 days after first intake. Persistence after cessation was not significantly different for R0052, HA-108, and HA-129 (~3–6 days), but B. longum R0175 persisted significantly longer (15–30 days) in a subset of 6 of 13 Intermediate WGTT participants, associated with a permissive microbiota profile characterized by higher Blautia, Roseburia, Parabacteroides, and Eggerthella. Limitations include small sample size, uncontrolled diet, transit time measured only during the first 3 days of intake, and use of qPCR rather than viable bacteria counts.
