Two rare flavonoid glycosides from Litsea glutinosa (Lour.) C. B. Rob.: experimental and computational approaches endorse antidiabetic potentiality

BMC Complementary Medicine and Therapies · 7 authors, 4 centres

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This study isolated two flavonoid glycosides from Litsea glutinosa leaves and tested a methanol extract in mice with streptozotocin-induced diabetes. The extract significantly reduced fasting blood glucose levels, and computational analysis suggested the isolated compounds may inhibit key enzymes involved in diabetes.

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The study isolated two flavonoid glycosides from Litsea glutinosa leaves. A methanolic extract was tested in streptozotocin-induced diabetic mice. Doses of 300 and 500 mg/kg/day significantly decreased fasting blood glucose levels starting from day 7 (p < 0.05), with the 500 mg/kg dose showing an effect comparable to metformin. Computational docking suggested the isolated compounds have strong binding affinities to human pancreatic α-amylase and aldose reductase, potentially inhibiting these diabetes-related enzymes.

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other

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endocrinology

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ORIGINAL PAPER

PMC10832099

Two rare flavonoid glycosides from Litsea glutinosa (Lour.) C. B. Rob.: experimental and computational approaches endorse antidiabetic potentiality

BMC Complementary Medicine and Therapies

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ABSTRACT

Background Litsea glutinosa (Lour.) C. B. Rob. belongs to the Litsea genus and is categorized under the family of Lauraceae . The study aimed to investigate the phytoconstituents and pharmacological properties of methanol extract of leaves of Litsea glutinosa , focusing on antidiabetic activity via in vivo and in silico techniques. Methods Extensive chromatographic and spectroscopic techniques were applied to isolate and characterize the constituents from the L. glutinosa plant species. The antidiabetic activity was studied in streptozotocin-induced diabetes mice, and the computational study of the isolated compounds was carried out by utilizing AutoDock Vina programs. In addition, the pharmacokinetic properties in terms of absorption, distribution, metabolism and excretion (ADME) and toxicological profiles of the isolated compounds were examined via in silico techniques. Results In the present study, two flavonoid glycosides 4΄- O -methyl (2 ˝,4 ˝-di- E -p-coumaroyl) afzelin (1) and quercetin 3- O -(2 ˝,4 ˝-di- E -p-coumaroyl)-α-L-rhamnopyranoside (2) were isolated from the leaves of L. glutinosa and characterized by 1 H and 13 C NMR, COSY, HSQC, HMBC, and mass spectral data. Although compounds 1 and 2 have been reported twice from Machilis litseifolia and Lindera akoensis , and Machilis litseifolia and Mammea longifolia , respectively, this is the first report of this isolation from a Litsea species. Administering the methanolic extract of L. glutinosa at doses of 300 and 500 mg/kg/day to mice with diabetes induced by streptozotocin led to a significant decrease in fasting blood glucose levels ( p < 0.05) starting from the 7th day of treatment. Besides, the computational study and PASS analysis endorsed the current in vivo findings that the both isolated compounds exerted higher binding affinities to human pancreatic α-amylase and aldose reductase than the conventional drugs. The in silico ADMET analysis revealed that the both isolated compounds have a favorable pharmacokinetic and safety profile suitable for human consumption. Conclusion According to the current outcomes obtained from in vivo and in silico techniques, the leaf extract of L. glutinosa could be a natural remedy for treating diabetes, and the isolated phytoconstituents could be applied against various illnesses, mainly hyperglycemia. However, more investigations are required for extensive phytochemical isolation and pharmacological activities of these phytoconstituents against broader targets with exact mechanisms of action. Supplementary Information The online version contains supplementary material available at 10.1186/s12906-024-04337-0.

CITATION EXPORT

Israt Jahan Bulbul, Md. Jamal Hossain, Mohammad Rashedul Haque et al.. Two rare flavonoid glycosides from Litsea glutinosa (Lour.) C. B. Rob.: experimental and computational approaches endorse antidiabetic potentiality. BMC Complementary Medicine and Therapies. (2024). https://doi.org/10.1186/s12906-024-04337-0

AUTHORS

7 · 4 CENTRES

Israt Jahan Bulbul, Md. Jamal Hossain, Mohammad Rashedul Haque, Muhammad Abdullah Al-Mansur, Choudhury M. Hasan, Abdullah Al Hasan, Mohammad A. Rashid

1https://ror.org/00cf0ab87grid.443031.10000 0004 0371 4375Department of Pharmacy, Southeast University, 252, Tejgaon Industrial Area, Dhaka 1208 Bangladesh
2https://ror.org/01g14tq52grid.443034.40000 0000 8877 8140Department of Pharmacy, School of Pharmaceutical Sciences, State University of Bangladesh, South Purbachal, Kanchan, Dhaka, 1461 Bangladesh
3https://ror.org/05wv2vq37grid.8198.80000 0001 1498 6059Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, 1000 Bangladesh
4https://ror.org/03njdre41grid.466521.20000 0001 2034 6517Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka, 1205 Bangladesh