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other·preclinical research, biomedical research, nutrition, antioxidants·PMC10608729

Unlocking Potentially Therapeutic Phytochemicals in Capadulla ( Doliocarpus dentatus ) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics

Metabolites · 4 authors, 4 centres

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TL;DR

This study used untargeted mass spectrometry to identify phytochemical compounds in red and white ecotypes of the Guyanese plant Doliocarpus dentatus (Capadulla).

Full summary

677 CHARS

The Orbitrap high-resolution mass spectrometer provided high resolving power and broad analyte coverage, with identification performed via spectral library matching and database queries. The analysis identified numerous polyphenolic compounds, including epicatechin methyl gallate, catechin gallate, proanthocyanidin isomers, quercetin 3 glycoside, apigenin, catechin, and resveratrol, among others. The authors note that future work will include antioxidant assays and bioactivity testing on cell lines. The implications are limited to identifying potential compounds for future research into conditions linked to oxidative stress, but no efficacy or mechanism is established.

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other

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preclinical research

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AI pipeline

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100% · A

ORIGINAL PAPER

PMC10608729

Unlocking Potentially Therapeutic Phytochemicals in Capadulla ( Doliocarpus dentatus ) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics

Metabolites

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ABSTRACT

Abstract Doliocarpus dentatus is thought to have a wide variety of therapeutic phytochemicals that allegedly improve libido and cure impotence. Although a few biomarkers have been identified with potential antinociceptive and cytotoxic properties, an untargeted mass spectrometry-based metabolomics approach has never been undertaken to identify therapeutic biofingerprints for conditions, such as erectile dysfunction, in men. This study executes a preliminary phytochemical screening of the woody vine of two ecotypes of D. dentatus with renowned differences in therapeutic potential for erectile dysfunction. Liquid chromatography–mass spectrometry-based metabolomics was used to screen for flavonoids, terpenoids, and other chemical classes found to contrast between red and white ecotypes. Among the metabolite chemodiversity found in the ecotype screens, using a combination of GNPS, MS-DIAL, and SIRIUS, approximately 847 compounds were annotated at levels 2 to 4, with the majority of compounds falling under lipid and lipid-like molecules, benzenoids and phenylpropanoids, and polyketides, indicative of the contributions of the flavonoid, shikimic acid, and terpenoid biosynthesis pathways. Despite the extensive annotation, we report on 138 tentative compound identifications of potentially therapeutic compounds, with 55 selected compounds at a level-2 annotation, and 22 statistically significant therapeutic biomarkers, the majority of which were polyphenols. Epicatechin methyl gallate, catechin gallate, and proanthocyanidin A2 had the greatest significant differences and were also relatively abundant among the red and white ecotypes. These putatively identified compounds reportedly act as antioxidants, neutralizing damaging free radicals, and lowering cell oxidative stress, thus aiding in potentially preventing cellular damage and promoting overall well-being, especially for treating erectile dysfunction (ED). Keywords: Capadulla, erectile dysfunction, mass spectrometry, metabolomics, polyphenols, antioxidants

CITATION EXPORT

Ewart Smith, Ainsely Lewis, Suresh S Narine et al.. Unlocking Potentially Therapeutic Phytochemicals in Capadulla (Doliocarpus dentatus) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics. Metabolites. (2023). https://doi.org/10.3390/metabo13101050

AUTHORS

4 · 4 CENTRES

Ewart Smith, Ainsely Lewis, Suresh S Narine, R J Neil Emery

1Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON K9J 0G2, Canada
2Department of Biology, Trent University, Peterborough, ON K9J 0G2, Canada
3Trent Centre for Biomaterials Research, Trent University, Peterborough, ON K9J 0G2, Canada
4Departments of Physics & Astronomy and Chemistry, Trent University, Peterborough, ON K9J 0G2, Canada