Xylanopectinolytic enzymes by marine actinomycetes from sediments of Sarena Kecil, North Sulawesi: high potential to produce galacturonic acid and xylooligosaccharides from raw biomass

Journal of Genetic Engineering & Biotechnology · 7 authors, 4 centres

AI SUMMARY

FIDELITY 100%

This summary was generated by AI from a single paper. It has not been reviewed by a clinician and is not clinical advice. Verify against the source before acting on it.

AGENT 01 · STRUCTURED SUMMARY

TRACE TO SOURCE

“

TL;DR

Pectinase and xylanase enzymes from marine actinomycete isolate BLH 5-14, identified as Streptomyces tendae, were characterized.

Full summary

424 CHARS

Pectinase and xylanase enzymes from marine actinomycete isolate BLH 5-14, identified as Streptomyces tendae, were characterized. Pectinase showed highest activity of 1.44±0.08 U/mL on day 6 at pH 8.0 and 50°C, producing galacturonic acid. Xylanase showed maximum activity of 4.33±0.03 U/mL on day 6 at pH 6.0 and 60°C, yielding xylooligosaccharides. Both enzymes were active across pH 3.0-10.0 and temperatures 30°C to 70°C.

STUDY TYPE

other

SPECIALTY

preclinical research

SUMMARISED BY

AI pipeline

FIDELITY CHECK

100% · A

ORIGINAL PAPER

PMC10017887

Xylanopectinolytic enzymes by marine actinomycetes from sediments of Sarena Kecil, North Sulawesi: high potential to produce galacturonic acid and xylooligosaccharides from raw biomass

Journal of Genetic Engineering & Biotechnology

READ THE FULL PAPER

ABSTRACT

Abstract Background Actinomycetes isolated from marine habitats are known to have the potential for novel enzymes that are beneficial in the industry. In-depth knowledge is necessary given the variety of this bacterial group in Indonesia and the lack of published research. Actinomycetes isolates (BLH 5-14) obtained from marine sediments of Sarena Kecil, Bitung, North Sulawesi, Indonesia, showed an ability to produce pectinase and xylanase that have equal or even higher potential for pectic-oligosaccharides (POS) and xylooligosaccharides (XOS) production from raw biomass than from commercial substrates. This study's objective was to characterize both enzymes to learn more for future research and development. Results Pectinase had the highest activity on the 6 th day (1.44±0.08 U/mL) at the optimum pH of 8.0 and optimum temperature of 50 °C. Xylanase had the maximum activity on the 6 th day (4.33±0.03 U/mL) at optimum pH 6.0 and optimum temperature 60 °C. Hydrolysis and thin layer chromatography also showed that pectinase was able to produce monosaccharides such as galacturonic acid (P1), and xylanase was able to yield oligosaccharides such as xylotriose (X3), xylotetraose (X4), and xylopentaose (X5). BLH 5–14 identified as the genus Streptomyces based on the 16S rDNA sequences and the closely related species Streptomyces tendae (99,78%). Conclusions In the eco-friendly paper bleaching industry, Streptomyces tendae has demonstrated the potential to create enzymes with properties that can be active in a wide range of pH levels. The oligosaccharides have the potential as prebiotics or dietary supplements with anti-cancer properties. Further research is needed to optimize the production, purification, and development of the application of pectinase and xylanase enzymes produced by Actinomycetes isolates. Keywords: Actinomycetes, Enzyme characterization, Galacturonic acid, Marine sediment, Pectinase, Pectic-oligosaccharides, Xylanase, Xylooligosaccharides

CITATION EXPORT

Hana Nadhifah, Nanik Rahmani, Wibowo Mangunwardoyo et al.. Xylanopectinolytic enzymes by marine actinomycetes from sediments of Sarena Kecil, North Sulawesi: high potential to produce galacturonic acid and xylooligosaccharides from raw biomass. Journal of Genetic Engineering & Biotechnology. (2023). https://doi.org/10.1186/s43141-023-00488-8

AUTHORS

7 · 4 CENTRES

Hana Nadhifah, Nanik Rahmani, Wibowo Mangunwardoyo, Yopi, Akhirta Atikana, Shanti Ratnakomala, Puspita Lisdiyanti

1Biology Department, Faculty of Mathematics and Natural Sciences, University of Indonesia, Pondok Cina, Depok, West Java 16424 Indonesia
2Research Center for Applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, KM. 46, Cibinong, Bogor, West Java, 16911 Indonesia
3Deputy of Regional Research and Innovation Agency, National Research and Innovation Agency, Jl. M.H. Thamrin No.8, Jakarta, 10340 Indonesia
4Research Center for Biosystematics and Evolution, Organization Research of Life Sciences and Environment, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, KM. 46, Cibinong, Bogor, West Java, 16911 Indonesia